.

Дослідження медулярних механізмів участі оксиду азоту та рецепторів N-метил-D-аспартату в регуляції дихального ритмогенезу у новонароджених щурят: Авт

Язык: украинский
Формат: реферат
Тип документа: Word Doc
115 2731
Скачать документ

IAOe?IIAEUeIA AEAAeAI?ss IAOE OE?A?IE

?INOEOOO O?C?IEIA?? ?i. I.I. AIAIIIEUeOess

——————————————————————–

Aiea?i Aeaien A?eoi?iae/

OAeE
612.2:612.821:616.24

AeINE?AeAEAIIss IAAeOEss?IEO IAOAI?CI?A O*ANO? IENEAeO ACIOO OA

?AOeAIOI??A N-IAOEE-D-ANIA?OAOO A ?AAOEssOe?? AeEOAEUeIIAI

?EOIIAAIACO O IIAIIA?IAeAEAIEO UO?ssO

03.00.13 – O?c?ieia?y ethaeeie ? oaa?ei

Aaoi?aoa?ao aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa a?ieia?/ieo iaoe

Eeaa – 1999

Aeena?oaoe??th ? ?oeiien

?iaioo aeeiiaii o a?aeae?e? ii aea/aiith a?iiene/ieo noai?a

?inoeoooo o?c?ieia?? ?i. I.I. Aiaiiieueoey IAI Oe?a?ie

Iaoeiaee ea??aiee: aeieoi? iaaee/ieo iaoe, i?ioani?

NA?AAeAIEI Ieoaeei Ieoaeeiae/

?inoeooo o?c?ieia?? ?i. I.I. Aiaiiieueoey IAI Oe?a?ie

caa?aeoaa/ a?aeae?eo ii aea/aiith a?iiene/ieo noai?a

Io?oe?ei? iiiiaioe: aeieoi? a?ieia?/ieo iaoe OAIIAAE Ethaeieea
Ieeiea?aia

?inoeooo o?c?ieia?? ?i. I.I. Aiaiiieueoey IAI Oe?a?ie

i?ia?aeiee iaoeiaee ni?a?ia?oiee a?aeae?eo

o?c?ieia?? e?iaiia?ao

aeieoi? iaaee/ieo iaoe NIEIAEIA Aiaoie?e ?aaiiae/

?inoeooo oa?iaeieia?? oa oieneeieia?? AII Oe?a?ie

aieiaiee iaoeiaee ni?a?ia?oiee a?aeae?eo

aenia?eiaioaeueii? oa?ai??

I?ia?aeia onoaiiaa: eaoaae?a o?c?ieia?? ethaeeie oa oaa?ei Ee?anueeiai

oi?aa?neoaoo ?iai? Oa?ana Oaa/aiea

Caoeno a?aeaoaeaoueny “_27_” “_ea?oiy_ ” 1999 ?. i “_14__” aiaeei?

ia can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae 26.198.01 i?e ?inoeooo?

o?c?ieia?? ?i. I.I. Aiaiiieueoey IAI Oe?a?ie ca aae?anith: 252024

Ee?a, aoe. Aiaiiieueoey, 4.

C aeena?oaoe??th iiaeia iciaeiieoeny o a?ae?ioaoe? ?inoeoooo o?c?ieia??

?i. I.I. Aiaiiieueoey

Aaoi?aoa?ao ?ic?neaiee “24” “_ethoiai” 1999 ?.

A/aiee nae?aoa? niaoe?ae?ciaaii? a/aii? ?aaee

aeieoi? a?ieia?/ieo iaoe C.I. Ni?ie?ia-Ia??ia

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue oaie. Iaiao?aei?noue aeine?aeaeaiiy oeaio?aeueii?
?aaoeyoe?? aeeoaeueiiai ?eoiiaaiaco o iiaiia?iaeaeaieo nnaaoe?a
iia’ycaia iiiaae ona c oei, ui a no?oeoo?? i?e/ei nia?oiino? ae?oae a
?aiiueiio iaiiaoaeueiiio ia?eiae? aeeoaeuei? ?iceaaee caeiathoue
i?ia?aeia i?noea. E??i oiai, a aeooa?nuee?e oa iaiiaoieia?/i?e
i?aeoeoe? aeei?enoiaothoueny oa?iaeieia?/i? i?aia?aoe, ye? iiaeooue
aooe aeiii?aie ieneaeo acioo (NO) ? aeieaoi?aie ?aoeaioi??a
N-iaoee-D-ania?oaoo (NMDA) (Ia?eiaa, Oaaaeia, 1993). A?aeiii, ui NO o
ia?aia?e nenoai? aeeiio? ooieoe?th ?ao?ia?aaeiiai ianaiaeaea?a,
iiaeoeyoi?a ye caoaeaeoth/i?, oae ? aaeuei?aii? neiaioe/ii?
ia?aaea/? (Dawson & Snyder, 1994; Wu & Dun, 1996), iieacaii eiai
ni?iiiaei?noue aei aeieoaaiiy iioaioe?aecaeaaeieo eaeueoe??aeo eaiae?a
iae?ii?a (Nieiaeiaa, Einothe, 1998). Iiaeeeaei oeyoii noeioeyoe??
aeoeaiino? oa?iaioo, ui a?ae-iia?aea? ca a?ineioac NO (NO-neioace), ?
ca?eueoaiiy aioo??oiueiee?oeiii? eiioeaio?aoe?? ?ii?a eaeueoe?th
aiane?aeie aeoeaaoe?? NMDA-?aoeaioi??a (Dawson & Snyder, 1994). Ia
oaia??oi?e /an o e?oa?aoo?? a?aenooi? aeai? i?i oeaio?aeueiee aieea NO
ia aeeoaeueiee ?eoi o iiaiia?iaeaeaieo oaa?ei, ye o ii?i?, oae ? ca
oiia a?iien??. Iaeiei ?c caaaeueiiaeciaieo iaoiae?a eieae?caoe??
iae?ii?a, ye? i?noyoue NO-neioaco, ? a?noio?i?/ia ?aeaioeo?eaoe?y
ia?ea?iiai oa?iaioo i?eioeiai?ae aaeai?i aeeioeeaioeae oinoao
(NADPH)-ae?aoi?ace (Dawson et al., 1991; Vincent & Kimura, 1992).
NADPH-ae?aoi?aci?aaeoeai? oa NO-neioaci?ioii?aaeoeai? iae?iie
eieae?ciaai? o aei?ineeo oaa?ei o iaaeoey?ieo no?oeoo?ao, i?e/aoieo aei
aaia?aoe?? aeeoaeueiiai ?eoio (Vincent & Kimura, 1992; Dun et
al.,1994). Aiaeia?/i? ?iaioe, ye? aoee a i?iaaaeai? ia iiaiia?iaeaeaieo
oaa?eiao, iaa?aeii?. ?ieue NMDA-?aoeaioi??a o ?aaoeyoe?? aeeoaeueiiai
?eoio o iiaiia?iaeaeaieo oaa?ei ia iaaeoey?iiio ??ai? caeeoa?oueny ia
inoaoi/ii c’yniaaiith. A ae-nia?eiaioao ia c??cao aeiaaanoiai iiceo
iiaiia?iaeaeaieo uo?yo io?eiai? aeai? i?i oa, ui NMDA-?aoeaioi?e ia
aa?ooue o/ano? a aeeoaeueiiio ?eoiiaaiac? (Funk et al., 1993).
Iaiao?aeii i?aee?aneeoe, ui oe? aeine?aeaeaiiy i?iaiaeeeeny a
?ic/ei? c i?aeaeuaiith ao?e/? eiioeaio?aoe??th ?ii?a eae?th,ui iiaea
i?ecaiaeeoe aei iioaioe?ae- aai eaeueoe?ecaeaaeii? aeanaineoecaoe??
NMDA-?aoeaioi??a (McBain & Mayer, 1994). ?acii c oei a?aeiii, ui
aie?eaoe?y aeciaaiiiai NMDA i?ecaiaeeoue aei noeioeyoe?? aeeoaeueiiai
?eoio, yeee aaia?o?oueny iaaeoeini?iaeueieie i?aia?aoaie
iiaiia?iaeaeaieo uo?yo (Greer et al., 1991; Kashiwagi et al., 1993).
I?ioe??//y ?nioth/eo aeaieo na?ae/aoue i?i iaiao?aei?noue i?iaeiaaeaiiy
aeine?aeaeaiue o aeaiiio iai?yieo. A?aeiii, ui ca a?iiene/ieo oiia
a?aeaoaa?oueny ca?eueoaiiy aea?eueiaiiy aeooaiaoo, ui iiaea i?ecaiaeeoe
aei aeoeaaoe?? NMDA-?aoeaioi??a (Mitiani at al., 1991; Johnston, 1997).
O/anoue NMDA-?aoeaioi??a o oeaio?aeuei?e ?aaoeyoe?? aeeoaeueiiai
?eoio o iiaiia?iaeaeaieo i?e a?iien?? i?e oeueiio ia ciaeoei
aeinoaoiueiai a?aeia?aaeaiiy a e?oa?aoo?ieo aeaea?aeao. Aeciaii, ui
a?aeoeeaiiy eiioeaio?aoe?? ?ii?a aiaeith aeey iaaeoey?ii?
oaii/ooeeaino? aeeoaiiy ? a?eueo neeueiei iiae?acieeii, i?ae a?aeoeeaiiy
aaciaiai neeaaeo (Eaeueiainii, 1997). Ca iiaeyaeii ?yaeo aeine?aeiee?a
(Cohen, 1979; Arita et al., 1988), oeaio?aeuei? oaii?aoeaioi?e
aaio?aeueii? iiaa?oi? aeiaaanoiai iiceo iaaeathoue caoaeaeoth/iai
oii?/iiai aieeao ia ?ini??aoi?i? iae?iie ?eoiiaaia?oth/i? ia?aae?.
I?eionea?oueny, ui NMDA-?aoeaioi?e iiaeooue a?aoe o/anoue o
caoaeaeo/?e neiaioe/i?e ia?aaea/? ia oeyoao, ye? a?aeiia?aeathoue ca
iiaeoeyoe?th caoaeeeaino? ?eoiiaaia?oth/eo iae-?ii?a (Funk et al.,
1993). Aea cia/aiiy ?aoeaioi??a aeaiiai oeio oa aiaeiaaiiiai ieneaeo
acioo a iaaeoey?i?e oaii/ooeeaino? aeeoaiiy ia aea/aii.

Iaoa aeine?aeaeaiiy iieyaaea o aecia/aii? ?ie? NO oa
NMDA-?aoeaioi??a caoaeaeoth/eo ai?iieeneio o ?aaoeyoe?? aeeoaeueiiai
?eoio iiaiia?iaeaeaieo uo?yo ia iaaeoey?iiio ??ai?.

Aieiai? caaea/?:

1. Aeine?aeeoe NADPH-ae?aoi?acio ?aaeoeai?noue iae?ii?a
iaaeoey?ieo no?oeoo?, i?e/aoieo aei aeeoaeueiiai ?eoiiaaiaco, o
iiaiia?iaeaeaieo uo?yo.

2. Aea/eoe aieea NO oa aeieoaaiiy NMDA-?aoeaioi??a ia aeaeo?e/io
aeoeai?noue o ae?ao?aaiaeueiiio ia?a? /anoeiai ecieueiaaieo
iaaeoeini?iaeueiuo i?aia?ao?a iiaiia?iaeaeaieo uo?yo (*?INI).

3. Aecia/eoe ?ieue NO o ci?i? aeaeo?e/ii? aeoeaiino? o
ae?ao?aaiaeueiiio ia?a? *?INI i?ae /an aieeao NMDA.

4. Aeine?aeeoe aieea NO oa aeieoaaiiy NMDA-?aoeaioi??a ia
aeaeo?e/io aeoeai?noue o ae?ao?aaiaeueiiio ia?a? *?INI i?e
a?iien?? oa i?e cieaeaii? ?I ciai?oiueiee?oeiii? ??aeeie.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Aia?oa aeine?aeaeaii
?iciiae?eaiiy iae?ii?a, ye? i?noyoue NADPH-ae?aoi?aco (a yeino? ia?ea?o
NO-neioace), oa ?o na?aaeith u?euei?noue a yae?ao aaio?aeueii? ?
aei?naeueii? ?ani??aoi?ieo a?oi iae?ii?a (A?A e Ae?A) aeiaaanoiai iiceo
iiaiia?iaeaeaieo uo?yo. Iieacaii i?enooi?noue i?/aieo ee?oei a yae?ao
A?A oa Ae?A, ?o iiioeyoe?th c iaea?eueoith u?euei?noth aeyaeaii a
?ino?aeuei?e /anoei? A?A – a iaeano? eaoa?aeueiiai ia?aa?aaioiee?oeiiiai
?aoeeoey?iiai yae?a. Aia?oa i?iaeaiiino?iaaii o/anoue NO a ?aaoeyoe??
aeeoaeueiiai ?eoio, yeee aaia?o?oueny *?INI iiaiia?iaeaeaieo uo?yo,
aeine?aeaeaii aeaye? iaoai?cie oe??? o/ano?. Aia?oa iieacaii ?ieue
aiaeiaaiiiai NI a oii?/iiio oi?iiciiio eiio?ie? aeeoaeueiiai ?eoio,
yeee cae?enith?oueny ?ino?aeueiith /anoeiith aaio?ieaoa?aeueiiai
a?aeae?eo aeiaaanoiai iiceo (AEAAeI) iiaiia?iaeaeaieo uo

?yo, o ii?i? ? ca oiia a?iiene/iiai oanoo. I?aeoaa?aeaeaii o/anoue
aiaeiaaiiiai NO a iiina?aaeeoaaii? aieeao aeoeaaoe?? NMDA-?aoeaioi??a
ia aeeoaeueiee ?eoi *?INI. Aia?oa aeine?aeaeaii ?ieue aiaeiaaiiiai NO a
iaaeoey?i?e oaii/ooeeaino? aeeoaiiy; iieacaii, ui oeae iae?iiiaeoeyoi?
iiina?aaeeiao? aeoeaaoe?th aeeoaeueiiai ?eoio *?INI i?e cieaeaii? ?I
noia?oocoth/iai ?ic/eio. Aia?oa aeine?aeaeaii aieea aeciaaiieo aeiii??a
NO ia aeeoaeueiee ?eoi *?INI. Iieacaii ca?eueoaiiy o?eaaeino?,
aiie?ooaee oa ?ioaa?aeueii? ?ioaineaiino? ?ini??aoi?ieo ?ic?yae?a (??)
c iaeii/aniei ciaioaiiyi ?o /anoioe i?e aie?eaoe?? aeiii??a NO.
I?iaeaiiino?iaaii ni?iiiaei?noue i?o?ii?oneaeo iao??th aei aeieoaaiiy
NMDA-?aoeaioi??a, ui aa?ooue o/anoue a ?aaoeyoe?? aeeoaeueiiai ?eoio
*?INI. Ca oiia a?iiene/iiai oanoo iiia?aaeiy aie?eaoe?y i?o?ii?oneaeo
iao??th i?ecaiaeeea aei a?eueo ae?aaeaiiai ca?eueoaiiy ?ioaa?aeueii?
?ioaineaiino? ?? ? iaioiai a?aeiiniiai i?eai?/aiiy ?o /anoioe, ui ?
i?aeoaa?aeaeaiiyi ?acoeueoao?a aenia?eiaio?a ?ioeo aeine?aeiee?a,
i?iaaaeaieo in vivo ia aei?ineeo oaa?eiao. O oeae aea /an aie?eaoe?y
i?o?ii?oneaeo iao??th i?e ianooiiiio aieea? ?ic/eio c? cieaeaiei ?I
ciaiooaaea noeioeyoe?th /anoioe aeeoaeueiiai ?eoio *?INI. Iaea?aeai?
?acoeueoaoe ?icoe?ththoue ?nioth/? oyaeaiiy i?i o/anoue NMDA-?aoeaioi??a
o ?aaoeyoe?? aeeoaeueiiai ?eoio ia iaaeoey?iiio ??ai? o
iiaiia?iaeaeaieo oaa?ei. Aia?oa iieacaii ca?eueoaiiy o?eaaeino? ?? o
ae?ao?aaiaeueiiio ia?a? *?INI i?e aie?eaoe?? eaoai?io, ui iiaea
na?ae/eoe i?i o/anoue NMDA-?aoeaioi??a o ia?aeeth/aii? aaeeoo ia aeaeeo
ia iaaeoey?iiio ??ai? o iiaiia?iaeaeaieo uo?yo. Oeae iaoai?ci
caaacia/o?oueny no?oeoo?aie ?ino?aeueii? /anoeie AEAAeI. Aia?oa
iieacaii o/anoue NMDA-?aoeaioi??a o oac? ei?ioei/anii? noeioeyoe??
aeeoaeueiiai ?eoio *?INI ca oiia a?iiene/iiai oanoo, a oaeiae o
a?eueo o?eaaeiio i?ioean? aeoeaaoe?? /anoioe aeeoaeueiiai ?eoio i?e
cieaeaii? ciai?oiueiee?oeiiiai ?I.

Oai?aoe/ia oa i?aeoe/ia cia/aiiy ?iaioe. ?acoeueoaoe
aeine?aeaeaiiy iathoue iaaio oai?aoe/io oe?ii?noue, ine?eueee
aeicaieythoue noi?ioaaoe iia? oyaeaiiy i?i iaaeoey?i? iaoai?cie o/ano?
NO ? NMDA-?aoeaioi??a o ?aaoeyoe?? aeeoaeueiiai ?eoio o
iiaiia?iaeaeaieo oaa?ei a ii?i?, a oaeiae i?e a?iien?? oa cieaeaii?
ciai?oiueiee?oeiiiai ?I, ye? a?aeaoaathoueny i?ae /an aaaaoueio
iaoieia?/ieo noai?a, o oiio /ene? ? aeeoaeueieo ?iceaae?a. I?aeoe/ia
cia/aiiy ?iaioe iieyaa? o aeaiiino?aoe?? iiaeeeaino? ci?ithaaoe
ia?aiao?e iaaeoey?iiai aeeoaeueiiai ?eoio iiaiia?iaeaeaieo oaa?ei oeyoii
oa?iaeieia?/iiai aieeao ia iaoai?cie eiai ?aaoeyoe?? ca o/anoth NO
oa NMDA-?aoeaioi??a.

Iniaenoee aianie caeiaoaa/a. Caeiaoaa/ai iniaenoi aeeiiaii anth
aenia?eiaioaeueio /anoeio, i?iaaaeaii aiae?c oa ocaaaeueiaiiy ??
?acoeueoao?a o ca’yceo c aeaieie e?oa?aoo?e. Caeiaoaa/ a?aa aeoeaio
o/anoue a iaaiai?aii? ?acoeueoao?a aenia?eiaio?a. Aia?oa caeiaoaa/ai
i?iaeaiiino?iaaii ?nioaaiiy o iiaiia?iaeaeaieo uo?yo ii?oieia?/iiai
aaceno o/ano? NO a ?aaoeyoe?? aeeoaeueiiai ?eoio, io?eiaii aeai? i?i
a??ia?aeiee aieea aiaeiaaiiiai NO oa aeciaaiieo eiai aeiii??a, a
oaeiae aeieoaaiiy NMDA-?aoeaioi??a ia ?ioaa?aeueio aeaeo?e/io
aeoeai?noue iaaeoey?iiai aeeoaeueiiai aaia?aoi?o. I?ia?aiia
caaacia/aiiy, ui ianeoaiao? aeaeo?io?c?ieia?/i? aenia?eiaioe,
?ic?iaeaia A.A. Oai?eii oa A.A. Ia?/aieii.

Ai?iaaoe?y aeena?oaoe??. Iniiai? iieiaeaiiy ?iaioe aeiiia?aeaeeny oa
iaaiai?thaaeeny ia XV C’?cae? Oe?a?inueeiai o?c?ieia?/iiai oiaa?enoaa
(Aeiiaoeuee, 1998), I?aeia?iaei?e eiioa?aioe?? “Hypoxia:
Destructive and Constructive Effect” (Ee?a, 1998), Anaoe?a?inuee?e
iaoeia?e eiioa?aioe??”I?iaeaie a?eiai? o?c?ieia??” (Eooeuee, 1998),
I?aeia?iaei?e eiioa?aioe?? “?ieue iiiiieneaea acioa a i?ioeannao
aeeciaaeayoaeueiinoe” (Ieine, 1998), I (onoaiia/?e) eiioa?aioe??
Oe?a?inueeiai oiaa?enoaa iae?iiaoe (Ee?a,1998),can?aeaiiyo a?aeae?eo
ii aea/aiith a?iiene/ieo noai?a ?inoeoooo o?c?ieia?? ?i. I.I.
Aiaiiieueoey IAI Oe?a?ie (1997,1998), can?aeaii? naeoi?o o?c?ieia??
a?noea?aeueieo nenoai ?inoeoooo o?c?ieia?? ?i. I.I. Aiaiiieueoey IAI
Oe?a?ie (1998).

Ioae?eaoe??. Ca ?acoeueoaoaie ?iaioe iioae?eiaaii /ioe?e noaoo? oa
oace i’yoe aeiiia?aeae.

No?oeoo?a oa ianya aeena?oaoe??. Aeena?oaoe?y neeaaea?oueny c?
anooio, iaeyaeo e?oa?aoo?e, iieno iaoiae?a aeine?aeaeaiiy, ?acoeueoao?a
aeine?aeaeaiiy, iaaiai?aiiy ?acoeueoao?a, aeniiae?a oa nieneo
aeei?enoaieo aeaea?ae ?c 177 iaeiaioaaiue. ?iaioo aeeeaaeaii ia 156
noi??ieao oa ?ethno?iaaii 22 ?enoieaie ? 5 oaaeeoeyie .

INIIAIEE CI?NO AeENA?OAOe??

Iaoa??aee oa iaoiaee aeine?aeaeaiue.

A?noio?i?/i? aeine?aeaeaiiy i?iaaaeai? ia 8 o?e-/ioe?eaeiaiaeo
uo?yoao e?i?? A?noa?. Aeyaeaiiy NADPH-ae?aoi?aci?aaeoeaieo (NADPH-ae)
no?oeoo? i?iaiaeeeiny ca aeiiiiiaith iiaeeo?eiaaiiai iaoiaeo
A?inaioa ? E?io?e (Vincent & Kimura, 1992; Petrovicky & Nemcova,
1995) ia o?iioaeueieo c??cao aeiaaanoiai iiceo oa ia?oeo o?ueio
naaiaio?a nieiiiai iiceo. I?ae aeeaieei ao??iei ia?eicii o oaa?ei
?ice?eaaee iicie, yeee i?ney 10-15 oa noia?ooc?? o?enaoi?ii (4-5%
ia?aoi?iaeueaeaa?aeo ia 0.1 I oinoaoiiio aooa??, pH 7.3) aeae?eyee ?
i?ioyaii 2 aiae i?iaiaeeee iinoo?enaoe?th i?e 4-50 N. I?ney 24 aiae
aeniiceoe?? a 15%-io ?ic/ei? naoa?ice o e??inoao? aeaioiaeyee c??ce 50
iei caaoiaoee. I?iaiaeeee a?noio?i?/io ?aaeoe?th o na?aaeiaeu? 0.1 I
oinoaoiiai aooa?o (pH 7.3), ui i?noeei 0.3% o?eoiio O-100, 0.5 ia/ie
aeaeeoiueiai oao?acie?oia, 1.0 ia/ie NADPH iao???ai? nie? oa 1.2 ia/ie
iaeaoo iao??th (an? ?aaeoeae ae?iaieoeoaa “Sigma,” NOA), i?e 37o N
i?ioyaii 40-60 oa. Aeine?aeaeaiiy c??c?a oa ?o i?e?ioioia?aooaaiiy
cae?enithaaee ca aeiiiiiaith na?oeiaiai i?e?ineiio (“Carl Zeiss,
Jena”, I?ia//eia). Eieae?caoe?th NADPH-ae-?aaeoeaieo ee?oei ia
noaiao o?iioaeueieo c??c?a i?iaiaeeee ca aeiiiiiaith
aeaioeii?aeeiaoiiai a?aoiiiaoae?aieea, iaae? yaea? eiio?iethaaeenue ca
aeiiiiiaith noa?aioaene/iiai aoeano (Paxinos & Watson, 1986).

Aeaeo?io?c?ieia?/i? aeine?aeaeaiiy. Aeey aeaioiaeaiiy *?INI in
situ (Suzue, 1984) aoei aeei?enoaii 167 o?e-/ioe?eaeiaiaeo uo?yo
e?i?? A?noa?. I?ae aeeaieei ao??iei ia?eicii i?iaiaeeee aei?naeueio
eai?iaeoiieth oa a?eaoa?aeueiee ia?a??c aei?naeueieo nieiiiiiceiaeo
ei??ioe?a. Aeae? ?ice?eaaee aaio?aeueio iiaa?oith noaieo iiceo, yeee
ia?a??caee ca ??aiai aeoiaeo ei??ioe?a n. abducens. A?aei?aia?iaoaaee
i?aaee ae?ao?aaiaeueiee ia?a. I?ney i?iaaaeaiiy aaio?aeueii?
eai?iaeoii?? nieiiee iicie ia?a??caee ca ??aiai N6-N7. O aeayeeo
aenia?eiaioaeueieo na??yo i?iaiaeeee iiia?a/i? ia?a??ce AEAAeI ca
?ino?aeueiith iaaeith ei??ioe?a IX-X ia? /a?aiiiiiceiaeo ia?a?a – i?ae
oaii/ooeeaeie ciiaie I ? S. Ca?aeii aeaiei e?oa?aoo?e (Ia?/aiei oa
?i., 1995), ia?a??ce ca oeei ??aiai i?ecaiaeyoue aei noeioeyoe??
/anoioe ?? o ae?ao?aaiaeueiiio ia?a? *?INI uo?yo ia?oeo aei?a aeeooy.
Aeaeo?e/io aeoeai?noue o ae?ao?aaiaeueiiio ia?a? (??) a?aeaiaeeee
a?iiey?ii, i?aeneethaaee (eiao?oe??io i?aeneeaiiy 104-105 ),
aeene?ei?ioaaee oa ?ioaa?oaaee ca aeiiiiiaith aiie?ooaeii-/anoioiiai
?ioaa?aoi?o c iino?eiith /ano 30.0 in. ?ioaa?iaai? neaiaee iiaeaaaee
ia 12-?ic?yaeiee aiaeiai-oeeo?iaee ia?aoai?thaa/ (AOeI), c’?aeiaiee c
ia?niiaeueiei eiii’thoa?ii.

Aeey noia?ooc?? aeei?enoiaoaaee iiaeeo?eiaaiee ?ic/ei E?aano
ianooiiiai neeaaeo (o i?e?iieyo ia 1 e ): NaCl – 124.0; KCl- 5.0;
KH2?I4 1.2; CaCl2 – 2.4; MgSO4- 1.3; NaHCO3 – 26.0; aetheica-30.0 (pH
7.4). O neeaae? oanooth/iai ?ic/eio c ?I 7.0 eiioeaio?aoe?th NaCl aoei
ca?eueoaii aei 140, a NaHCO3 – ciaioaii aei 10 i?e?iieue ia 1 e,
aeniiceoe?y aeaiiai ?ic/eio neeaaeaea 3 oa. ?ic/eie iane/oaaee
ea?aiaaiii (95% I2 + 5% NI2) i?ioyaii 10 oa i?e oaeaeeino? iiaea/?
aaciai? noi?o? 1 e/oa. A?iien?y iiaeaethaaeany aie?eaoe??th i?ioyaii
3 oa noia?oocoth/iai ?ic/eio, iane/aiiai ca oeo aea oiia aaciaith
noi?ooth, ui i?noeea 95% N2 ? 5% NI2. Oaeaee?noue noia?ooc?? neeaaeaea
4.0 ie/oa i?e 26.5 oN. I?ioyaii aenia?eiaio?a i?iaiaeeeiny aei??aiiy
?I2 ? ?I noia?oocoth/iai ?ic/eio, a oaeiae i?e?ii?ia ?ic/eio,
a?ae?a?aieo aaciina?aaeiuei ia aaio?aeuei?e iiaa?oi? aeiaaanoiai
iiceo, ca aeiiiiiaith i?e?iaiae?caoi?o (pH/Blood Gas Analyzer,
Type OP-215 “Radelkis”, Oai?ueia). O na?aaeiueiio ?I2 o ?ic/ei? iaae
iiceii neeaaeaei 431± 34 ii ?o. no. o eiio?ie? oa 36± 9 ii ?o.no. ca
oiia a?iiene/iiai oanoo. Na?aaei? cia/aiiy ?I ?ic/eio neeaaeaee
7.34±0.09 o eiio?ie?, 7.30±0.11 i?e a?iien?? oa 7.01±0.06 aeey
oanooth/iai ?ic/eio c? cieaeaiei ?I.

A ?iaio? canoinoaaee: 1) eiieo?aioiee ?ia?a?oi? NO-neioace
iaoeeiaee ao?? NG-i?o?i-L-a?a?i?io (L-NAME) o eiioeaio?aoe?yo 1.0 oa
10.0 ieIieue/e; 2) ae/a/ee aaiiaeia?i, aaaio, ui ca’yco? NO (Hb), o
eiioeaio?aoe?? 0.30 ieIieue/e; 3) aeciaaii? aeiii?e NO i?o?ii?oneae
iao??th (SNP) ? i?o?iae?oea?ei o eiioeaio?aoe?yo 1.0 oa 10.0
ieIieue/e; 4) L-a?a?i?i, noano?ao a?ineioaco NO, o eiioeaio?aoe?yo
1.0 oa 10.0 ieIieue/e; 5) aaii?no a?aeiia?aeiiai oeio ?aoeaioi??a
NMDA o eiioeaio?aoe?? 5.0 ieIieue/e (an? ?aaeoeae ae?iaieoeoaa
“Sigma”, NOA); 6)iaeiieo?aioiee aeieaoi? NMDA-?aoeaioi??a
2-(i?oi-oei?oai?e)-2-(iaoeeai?ii)-oeeeeiaaenai (eaoai?i, “Gedeon
Richter”, Oai?ueia) o eiioeaio?aoe?yo 1.0, 10.0 oa 100.0 ieIieue/e.

Oanooth/? aeniiceoe?? (a?iiene/iiai ? aoeeaeioe/iiai ?ic/ei?a,
?ic/eio, ui i?noea NMDA) neeaaeaee 3 oa. A ie?aieo aenia?eiaioaeueieo
na??yo?o i?iaiaeeee i?ney 10 oa noia?ooc?? ?ic/eiii, ui i?noea iaeei ?c
aeine?aeaeoaaieo i?aia?ao?a, aai ?o eiia?iaoe?th. ?a?no?aoe?th ??
i?iaiaeeee i?ioyaii 9 oa (3 oa eiio?ieth, 3 oa – noia?ooc?y oanooth/ei
?ic/eiii ? 3 oa – a?aeieaaiiy). Ioe?ithaaee na?aaei? iieacieee
o?eaaeino? ?? (O?, in), ?o /anoioe (Fi, oa-1), aiie?ooaee (A, oiiai?
iaeeieoe?) oa eioaa?aeueii? ?ioaineaiino? (?, oiiai? iaeeieoe?). Iaeia
oiiaia iaeeieoey a?aeiia?aea? /anoio? 5 Aoe i?e aiieeooae? 5 ieA
?ioaa?iaaiiai neaiaeo ca iaeei oeeee ii?ino eaiaeo AOeI. Io?eiai?
?acoeueoaoe ia?iaeyee noaoenoe/ii ca aeiiiiiaith t-e?eoa??th
No’thaeaioa ( Ieioea? e ae?., 1991).

?acoeueoaoe aeine?aeaeaiue.

Aeine?aeaeaiiy NADPH-ae-?aaeoeaiino? iae?ii?a iaaeoey?ieo no?oeoo?,
i?e/aoieo aei aaia?aoe?? aeeoaeueiiai ?eoio, o iiaiia?iaeaeaieo uo?yo.
Cia/io e?euee?noue NADPH-ae-?aaeoeaieo iae?ii?a aoei ciaeaeaii ia an?o
?ino?ieao aeaeueieo ??aiyo aeiaaanoiai iiceo. Ia ?ino?aeueieo ??aiyo
iaea?eueo? iiioeyoe?? iiceoeaieo ioeueoeiiey?ieo ee?oei na?aaeiueiai
ae?aiao?o (15-25 iei) aoee aeyaeai? o ?icoaoiaaieo aaio?aeueii yae?ao
?aoeeoey?ii? oi?iaoe??. Iaea?eueoo u?euei?noue i?/aieo iae?ii?a (o
na?aaeiueiio 27.9±2.6 ee?oeiia 0.1 ii2) aeyaeee o eaoa?aeueiiio
ia?aa?aaioiee?oeiiiio (LPG?), ?ino?iaaio?ieaoa?aeueiiio ?aoeeoey?iiio
(o na?aaeiueiio 14.2±2.1 ee?oei ia 0.1 ii2) oa a?aaioiee?oeiiiio
yae?ao (o na?aaeiueiio 12.67± 1.2 ee?oeiia 0.1 ii2). Ia a?eueo
eaoaeaeueieo ??aiyo NADPH-ae-?aaeoeai?noue iae?ii?a niinoa??aaee o
aaio?aeueieo iaeanoyo ia?aiaae?aiiiai ?aoeeoey?iiai yae?a (o
na?aaeiueiio 10.1± 1.3 ee?oei ia 0.1 ii2) oa aaio?aeueiiio iaaeoey?iiio
?aoeeoey?iiio yae?? (o na?aaeiueiio 4.3 ± 0.7 ee?oei ia 0.1 ii2).A?oia
i?/aieo iae?ii?a iaeiai oa na?aaeiueiai ae?aiao?o i?enooiy oaeiae o
i?ii?aeiiio ?aoeeoey?iiio yae?? oa o ?ao?ioaoe?aeuei?e iaeano?. O
iaithaeiiio yae?? ia an?o eiai ?ino?ieaoaeaeueieo ??aiyo i?enooi?
iiiaeeiie? iiceoeai? ee?oeie (o na?aaeiueiio 2.9±0.3 ee?oei ia 0.1
ii2). Iiiaeeiie? i?/ai? iae?iie aeyaeaii oaeiae ia eaoaeaeueieo ??aiyo
a iaeano? iicaiaithaeiiai yae?a. Iiceoeai? ioeueoeiiey?i? iae?iie
na?aaeiueiai aeeaiao?o niinoa??aaee ia an?o ?ino?ieaoaeaeueieo ??aiyo
a yae?? iiiaeeiieiai o?aeoo, iaea?eueoo ?o u?euei?noue (o na?aaeiueiio
6.2±0.4 ee?oei ia 0.1 ii2) aeyaeee o aaio?ieaoa?aeueieo eiai iaeanoyo.
Iiiaeeiie? i?/ai? iae?iie ciaeaeai? a yae?? i?ae’yce/iiai ia?ao oa ?o
a?oie – a iaeano? aei?naeueiiai iioi?iiai yae?a aeoeath/iai ia?ao.
Oaeei /eiii, o uo?yo ia?oeo aei?a aeeooy NADPH-ae-?aaeoeai? iae?iie
?niothoue a on?o yae?ao Ae?A oa A?A, ui iiaea neeaaeaoe ii?oieia?/io
iniiao o/ano? NO a oeaio?aeuei?e ?aaoeyoe?? aeeoaeueiiai ?eoiiaaiaco o
iiaiia?iaeaeaieo uo?yo.

Aieea NO oa aeieoaaiiy NMDA-?aoeaioi??a ia aeaeo?e/io aeoeai?noue o
ae?ao?aaiaeueiiio ia?a? *?INI. Aie?eaoe?y 10.0 ieIieue L-NAME i?ecaaea
aei a??ia?aeiiai ca?eueoaiiy /anoioe (ia 23.4%) c? ciaiueoaiiyi
o?eaaeino? (ia 12.7%), aiie?ooaee (ia 14.0%) oa ?ioaa?aeueii?
?ioaineaiino? ?? (ia 11.1%). Ci?ie aiaeia?/ii? ni?yiiaaiino?
niinoa??aaee i?e aie?eaoe?? aaiiaeia?io (oaae. 1). I?ney
a?aeie?aieaiiy ?ino?aeueii? /anoeie AEAAeI /anoioa ?? i?e aie?eaoe??
10.0 ieIieue L-NAME ciaiooaaeany ia 29.4% (oaae. 1). Aie?eaoe?y
10.0 ieIieue L-a?a?i?io aeeeeeaea i?ioeeaaei? ci?ieia?aiao??a ??: ?o
o?eaae?noue, aiie?ooaea oa ?ioaa?aeueia ?ioaineai?noue a??ia?aeii
ca?eueooaaeeny (ia 7.0%, 18.1% e 15.8% a?aeiia?aeii), a /anoioa
ciaiooaaeany ia 6,2%. I?ney a?aeie?aieaiiy ?ino?aeueii? /anoeie
AEAAeI aie?eaoe?y L-a?a?i?io, iai?ioe, aeeeeeaea ca?eueoaiiy /anoioe ??
(oaae.1). Aie?eaoe?y 10.0 ieIieue i?o?iae?oea?eio i?ecaaea aei
a??ia?aeiiai ca?eueoaiiy aiie?ooaee oa ?ioaa?aeueii? ?ioaineaiino? ??
( ia 13.0% oa 16.9% a?aeiia?aeii) oa ciaioaiiy /anoioe ia 26.2%. O
eiioeaio?aoe?? 10.0

ieIieue ?ioee aeiii? NO, SNP, oaeiae aeeeeeaa cieaeaiiy /anoioe (ia
21.1%) oa ca?eueoaiiy aiie?ooaee oa ?ioaa?aeueii? ?ioaineaiino? (ia
14.9% e 17% a?aeiia?aeii). I?ney a?aeie?aieaiiy ?ino?aeueii? /anoeie
AEAAeI SNP aeeeeeaa ca?eueoaiiy /anoioe ?? (oaae.1). Oaeei /eiii,
aie?eaoe?y aaaio?a, ui aieeaathoue ia a?ineioac NO (L-NAME, L-a?a?i?i),
ui ca’ycothoue aiaeiaaiiee NO (Hb), a oaeiae aeciaaiieo aeiii??a NO
(i?o?iae?oea?ei, SNP) i?ecaiaeeoue aei a??ia?aeieo ci?i na?aaei?o
ia?aiao??a ?ioaa?iaaii? aeaeo?e/ii? aeoea

Oaae. 1. Aieea NO ia ia?aiao?e ??, ui aaia?othoueny *?INI
iiaiia?iaeaeaieo uo?yo c ?ioaeoiei aeiaaanoei iiceii oa i?ney
a?aeie?aieaiiy ?ino?aeueii? /anoeie AEAAeI (ciie I) oeyoii iiia?a/iiai
ia?a??co.

Oiiae aeine?aeo Ti, in Fi, oa-1 A,
oi.iae. I, oi. iae.

Eiio?ieue 799±27 6.49±0.24 104.4±4.6
4549±210

Eiio?ieue i?ney ia?a??co 672±16 13.93±0.37 82.3±2.2 3403±93

L-NAME, 10.0 ieIieue 698±23* 8.01±0.23* 89.8±6.3* 4044±131*

Oa ae, i?ney ia?a??co 581±19 9.83±0.26* 81.1±2.6
3239±150

Hb, 0.3 ieIieue 790±37 7.61±0.26* 93.8±5.4*
4438±505

Oa ae, i?ney ia?a??co 678±48 13.18±0.31 81.5±5.9
3336±492

L-a?a?i?i, 10.0 ieIieue 855±38* 6.09±0.41 123.3±6.2*
5268±497*

Oa ae, i?ney ia?a??co 685±50 20.26±3.68* 88.3±6.0
3573±635

SNP, 10.0 ieIieue 871±40* 5.12±0.23* 120.0±6.8*
5322±450*

Oa ae, i?ney ia?a??co 712±38 15.6±1.07* 84.8±5.7
3574±459

I?ei?oea. C??i/eaie iicia/aii noaoenoe/io a??ia?aei?noue a?aei?i
(P

Нашли опечатку? Выделите и нажмите CTRL+Enter

Похожие документы
Обсуждение

Ответить

Курсовые, Дипломы, Рефераты на заказ в кратчайшие сроки
Заказать реферат!
UkrReferat.com. Всі права захищені. 2000-2020